 DescriptionOlympus, the world leader in objective-based TIRF microscopy, announces another significant leap forward in multicolor TIRF. The Olympus cell^TIRF illuminator offers four motorized channels for simultaneous image capture. Intuitive software control of TIRF parameters allows instant setting and confirmation of TIRF angle and seamless transition back and forth to widefield fluorescence. With the cell^TIRF illuminator, each laser wavelength is optimally focused and each angle is individually set, allowing different wavelengths to have the same penetration depth. Users can preset calculated penetration depths for all lasers with a single mouse click; the system will individually adjust each laser’s angle to simultaneously capture TIRF information from all channels. |
Features and Benefits
- The only 4 line system with true simultaneous acquisition
- Motorized control of TIRF angles
- Precise adjustment of incident angle
- Software calculation of penetration depth
- Software based laser attenuation
- Integrated Point FRAP optics for 1st laser line
- Seamless widefield/TIRF switching
- 6 dedicated TIRF lenses, world’s highest NA of 1.65
- 405 – 640nm laser systems
- Flexibility to select your optimal wavelength
- TIRF and widefield or 100% widefield with 340nm Transmission
- 25mm filter access for each laser line
- Adjustable field stop for each laser line
- Rigid single billet Aluminum frame
- Single molecule and membrane research
Specifications
TIRF illuminator |
Available laser Systems |
405nm, 445nm, 473nm, 488nm, 515nm, 532nm, 561nm, 594nm, 640nm |
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Laser coupling |
FC or FC8 connector |
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Field Number |
FN11.8 |
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Illuminator Body |
Single Billet Aluminum |
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Safety |
Integrated Interlock |
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Laser systems |
Wavelengths |
405nm, 445nm, 473nm, 488nm, 515nm, 532nm, 561nm, 594nm, 640nm |
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Power |
Laser system available up to 100mW |
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Attenuation |
Software control of laser intensity |
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Imaging Shutter |
High speed 1msec TTL shutters |
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Safety |
Integrated interlock |
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Epi-Fluorescence |
Light source |
100W Mercury, 75W Xenon, Metal halide. |
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Selection |
TIRF/Brightfield or Brightfield only with 340nm transmission |
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Field number |
FN22 |
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TIRF Objectives |
60X Objectives |
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PLANAPON60XOTIRFM |
PLANAPO 60X, NA 1.45, WD 0.1mm, Correction collar |
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APON60XOTIRFM |
APO 60X, NA 1.49, WD 0.1mm, Correction collar |
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100X Objective |
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PLAPO100XOTIRFM |
PLANAPO 100X, NA 1.45 WD 0.10mm |
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UAPON100XTIRF |
UAPON 100X, NA 1.49 WD 0.09mm, Correction collar |
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AP0100XO-HR-SP |
APO 100X, NA 1.65, WD 0.10mm |
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150X Objectives |
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UAPON150XOTIRF |
UAPO 150X, NA 1.45,WD 0.07mm,Correction collar |
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Immersion Oil |
Type-F |
Low Auto Fluorescence Immersion oil |
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High Refractive Index |
RI 1.78 Immersion oil for use with AP0100XO-HR-SP 1.65NA 100X objective |
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Coverslips |
High Refractive Index |
RI 1.78 glass coverslips for use with AP0100XO-HR-SP 1.65NA 100X objective |
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Compatible microscopes |
IX71, IX81 and IX81-ZeroDrift |
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