The FLUOVIEW FV3000 Series is designed to meet some of the most difficult challenges in modern science. With the high sensitivity and speed required for live cell and tissue imaging, the FV3000 also provides an intuitive and adaptable interface, and is capable of microplate imaging and complex screening protocols. The series supports complete workflows from live cell 2D-6D (x,y,λ,z,t,p) imaging through image processing, like deconvolution, and analysis. Particular attention has been paid to the needs of cell biology, cancer research, and stem cell research. The FV3000 is optimized for macro to micro imaging of cells, tissues and small organisms.
Features and Benefits
No Compromise between Speed and Field of View. Many high-speed scanning methods restrict the field of view, limiting their usefulness for examining large areas with multiple cells. The FV3000 Series’ resonant scanner maintains a full 1X field of view, even at a video rate of 30 frames per second. Additional speed is generated by clipping the Y axis, even at 438 frames per second.
The FV3000 Series employs Olympus’ TruSpectral detection concept. Based on patented* Volume Phase Hologram (VPH) transmission and an adjustable slit to control light, the spectral detection in FV3000 and FV3000RS is highly efficient, enabling users to select the detection wavelength of each individual channel to 1 nm.
Finding areas of interest in samples can be challenging. The confocal optical design of the FV3000 Series supports macro to micro imaging so users can quickly switch from low magnification overview observation with 1.25X objectives to high-magnification, detailed observation with up to 150X objectives. Users can employ image stitching at both macro and micro levels to generate overview images that show samples in context.
Olympus offers four high NA silicone immersion objectives that deliver excellent performance for live cell imaging. The refractive index of silicone oil (ne ≈ 1.40) is close to that of living tissue (ne ≈ 1.38), enabling high-resolution observations deep inside living tissue with minimal spherical aberration caused by refractive index mismatch. Silicone oil does not dry out or harden, so there is never a need to refill oil, making it ideal for extended time-lapse observations.
This oil immersion objective minimizes lateral and axial chromatic aberration in the 405 - 650 nm spectrum. Colocalization images are acquired reliably and images are measured with superior positional accuracy. The objective also compensates for chromatic aberration through near infrared up to 850 nm, making it the ideal choice for quantitative imaging.
A Z-drive guide installed near the revolving nosepiece combines high thermal rigidity with the stability of a wraparound structure to significantly reduce the impact of heat and vibration and improve the quality of time-lapse imaging.